Hi Ramashita,
I assume you are connecting your clients directly to the DB server? If so (and if it's possible) search through my posts, I've posted instructions on how to do this previously.
If not, you will need to run the Blue Prism Server service that you've setup on your application server as a domain account that has at the very least db_datareader and db_datawriter as memberships. I would also suggest the following:
bpa_ExecuteSP_DataSource_bpSystem
bpa_ExecuteSP_DataSource_custom
bpa_ExecuteSP_System
Hope this helps.
Steve